Insertion junction: LMJ.RY0402.215311_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.215311
Chromosome:chromosome 4
Location:1763936
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre04.g212200 (1 of 15) 3.4.21.72 - IgA-specific serine endopeptidase / Immunoglobulin A1 protease intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAACAACATTATTACATGGGTTTGAGTCCG
Internal bar code:AGGCGGGCGCACTCGGGACGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:909
LEAP-Seq percent confirming:99.8916
LEAP-Seq n confirming:3685
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:24

Suggested primers for confirmation by PCR

Suggested primer 1:GTCCCATTGCACAACACTTG
Suggested primer 2:CACTATGGTGGTACGATGCG