| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.215311 |
| Chromosome: | chromosome 17 |
| Location: | 6934966 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g746047 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCGGATTTCGATCGCTGAGTACCATCGC |
| Internal bar code: | GTACTTGCTACGTTTCTCCCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 815 |
| LEAP-Seq percent confirming: | 99.2317 |
| LEAP-Seq n confirming: | 6458 |
| LEAP-Seq n nonconfirming: | 50 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACACGGATGGACTGAAGTG |
| Suggested primer 2: | AGGGCTGCTGGACTTCTGTA |