| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.215320 |
| Chromosome: | chromosome 2 |
| Location: | 1565320 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g084800 | SPO11A | (1 of 1) K10878 - meiotic recombination protein SPO11 (SPO11); meoisis-related DNA topoisomerase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTACAAATGCGTGGAAGCCCGGCCTCACC |
| Internal bar code: | CCGGTGTCGTGACAGTCGTTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1030 |
| LEAP-Seq percent confirming: | 88.2734 |
| LEAP-Seq n confirming: | 11186 |
| LEAP-Seq n nonconfirming: | 1486 |
| LEAP-Seq n unique pos: | 89 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCTCATCGACGACCTGTAT |
| Suggested primer 2: | TACTCTCCCCCACTCTCCCT |