| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.215320 |
| Chromosome: | chromosome 14 |
| Location: | 1967467 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g621250 | (1 of 2) K09668 - glycosyltransferase-like protein LARGE (LARGE) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGCTCCGTGCAGGTGAGCAGGCGATGGA |
| Internal bar code: | TATTTAACGTTGCGTGCCCTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 177 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAGTGCCCACCAACAACTC |
| Suggested primer 2: | GCAAGAACAAGATCGTGCAA |