| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.215333 |
| Chromosome: | chromosome 16 |
| Location: | 4323237 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g667700 | (1 of 3) IPR000104//IPR005069 - Antifreeze protein, type I // Nucleotide-diphospho-sugar transferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACATAGAGGCTTGAAGAAATGTATGGTGG |
| Internal bar code: | CCACCTAGTGGCGCACCCCATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 785 |
| LEAP-Seq percent confirming: | 19.8209 |
| LEAP-Seq n confirming: | 2457 |
| LEAP-Seq n nonconfirming: | 9939 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGCGGTGGTCTTTGTTAT |
| Suggested primer 2: | AAGCTTGCGAACACCCATAC |