Insertion junction: LMJ.RY0402.215335_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.215335
Chromosome:chromosome 7
Location:1333919
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g322300 XPD4,RAD3D RAD3/XP-D family DNA-binding helicase; (1 of 1) K11273 - chromosome transmission fidelity protein 1 [EC:3.6.4.13] (DDX11, CHL1, CTF1) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGTCCCACGCAGCCGACAGTGCTTTCACAC
Internal bar code:CACTAGGCAAGGTCTTGCCGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:699
LEAP-Seq percent confirming:95.2546
LEAP-Seq n confirming:7688
LEAP-Seq n nonconfirming:383
LEAP-Seq n unique pos:76

Suggested primers for confirmation by PCR

Suggested primer 1:GCAAGAGTTGGTGGGGAATA
Suggested primer 2:TGTGCTGAGAAAACGAAACG