| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.215335 |
| Chromosome: | chromosome 7 |
| Location: | 1333945 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g322300 | XPD4,RAD3D | RAD3/XP-D family DNA-binding helicase; (1 of 1) K11273 - chromosome transmission fidelity protein 1 [EC:3.6.4.13] (DDX11, CHL1, CTF1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCCTTTGGCTGCCCCTGGCTTTGGGCGGA |
| Internal bar code: | GGAGGGACGCGATCGCGTACAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 766 |
| LEAP-Seq percent confirming: | 94.8486 |
| LEAP-Seq n confirming: | 2099 |
| LEAP-Seq n nonconfirming: | 114 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAAGAGTTGGTGGGGAATA |
| Suggested primer 2: | TGTGCTGAGAAAACGAAACG |