| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.215356 |
| Chromosome: | chromosome 2 |
| Location: | 1659491 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g085650 | (1 of 1) K03424 - TatD DNase family protein (tatD) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGCACGTGCTGCTCTGGAGCTTGCGAAGA |
| Internal bar code: | GCTAACAGTAGTGTCACCGCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 948 |
| LEAP-Seq percent confirming: | 97.2248 |
| LEAP-Seq n confirming: | 31985 |
| LEAP-Seq n nonconfirming: | 913 |
| LEAP-Seq n unique pos: | 145 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCATTTGTCCGCTAGGTTCC |
| Suggested primer 2: | GCAAGCAACAGAGTCATGGA |