| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.215362 |
| Chromosome: | chromosome 8 |
| Location: | 825204 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g360850 | (1 of 1) K18798 - protein AFG1 (AFG1, LACE1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAATTCTCACCTGCACCTCATCAAAGAAC |
| Internal bar code: | CCTTCAGCGACACGAGATGCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 79 |
| LEAP-Seq percent confirming: | 5.03759 |
| LEAP-Seq n confirming: | 67 |
| LEAP-Seq n nonconfirming: | 1263 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCACCAGACCCTTGAGAG |
| Suggested primer 2: | CACACACACCATCGCATACA |