| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.215362 |
| Chromosome: | chromosome 8 |
| Location: | 825207 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g360850 | (1 of 1) K18798 - protein AFG1 (AFG1, LACE1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGATGGGTGGGTGGCTGGGTGGGTAGGCG |
| Internal bar code: | GTGACTGCTCAGGGAGTTAGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 240 |
| LEAP-Seq percent confirming: | 99.7699 |
| LEAP-Seq n confirming: | 1301 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGAGGTGTGTGTGTGTGTG |
| Suggested primer 2: | CACACACACCATCGCATACA |