Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.215371 |
Chromosome: | chromosome_9 |
Location: | 7856441 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre09.g416850 | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GCCTGGCCGACCTGCGCCCCTTCCGGCGGG |
Internal bar code: | CGGGCCTCGGATCTCCTGCGTA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 952 |
LEAP-Seq percent confirming: | 99.4612 |
LEAP-Seq n confirming: | 26030 |
LEAP-Seq n nonconfirming: | 141 |
LEAP-Seq n unique pos: | 111 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCCTTGTTCTCCTACCCCTC |
Suggested primer 2: | GGCGTCCAAGACAAAGGTTA |