| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.215372 |
| Chromosome: | chromosome 16 |
| Location: | 6151550 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g675600 | (1 of 5) IPR000104//IPR001214 - Antifreeze protein, type I // SET domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGAAGCCGGCACCGACAGCCGCCGGCGAC |
| Internal bar code: | ATTCAGTGTGGTACAAGTACCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 908 |
| LEAP-Seq percent confirming: | 98.537 |
| LEAP-Seq n confirming: | 2088 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCCTCACCCACATCTTCGT |
| Suggested primer 2: | TACGGATCTGACGTGGATGA |