Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.215386 |
Chromosome: | chromosome_3 |
Location: | 2892650 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre03.g162850 | FAP292 | Flagellar Associated Protein | sense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | AAAGCCGCTGTGGAGGGCCGGCGTTACTAC |
Internal bar code: | TGGACGAGTATATAACCTACGA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 602 |
LEAP-Seq percent confirming: | 99.5209 |
LEAP-Seq n confirming: | 1454 |
LEAP-Seq n nonconfirming: | 7 |
LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TTCACTTCTTTGCATGACGC |
Suggested primer 2: | GCGCTGGTGTCATCAGTAGA |