| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.215442 |
| Chromosome: | chromosome 4 |
| Location: | 282349 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g217750 | (1 of 1) PTHR16017//PTHR16017:SF0 - GASTRULATION DEFECTIVE PROTEIN 1-RELATED // WD REPEAT-CONTAINING PROTEIN 70 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTTCTTCCGCCCTCTTCACTCTGGGCGAA |
| Internal bar code: | CATGAACCTGGAAACGGAGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 178 |
| LEAP-Seq percent confirming: | 98.0857 |
| LEAP-Seq n confirming: | 1076 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCATACCCACCTCAGCATCA |
| Suggested primer 2: | CTCCTAACACCCTCCCAACA |