| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.215444 |
| Chromosome: | chromosome 9 |
| Location: | 7052309 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g411400 | MOT45 | PF13 protein required for dynein inner/outer arm assembly in the | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAATCTCGGCAACAGAGTGAAGAGACGCG |
| Internal bar code: | GGTCCTACTCAACGCTTTTCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 271 |
| LEAP-Seq percent confirming: | 85.6421 |
| LEAP-Seq n confirming: | 4575 |
| LEAP-Seq n nonconfirming: | 767 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGAAGGAAAAGGCAATGGA |
| Suggested primer 2: | TGTTTACTGCCCTTCGTTCC |