| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.215457 |
| Chromosome: | chromosome 17 |
| Location: | 1313744 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g705836 | (1 of 587) 2.7.11.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase; Chlamydomonas-specific family W protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAGGCGCAGCGGCATTGGCGGTGGTGGC |
| Internal bar code: | GAATAACGATGCCCTGGGTTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2430 |
| LEAP-Seq percent confirming: | 99.4702 |
| LEAP-Seq n confirming: | 3567 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACAATCCTCCTTCCATGCC |
| Suggested primer 2: | GTGCGTCACCGTATGTCATC |