| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.215460 |
| Chromosome: | chromosome 12 |
| Location: | 337514 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g494500 | LF2,CDKI1 | Cyclin dependent kinase; (1 of 1) PTHR24056//PTHR24056:SF171 - CELL DIVISION PROTEIN KINASE // CYCLIN-DEPENDENT KINASE 20 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAAACATGCGTAATGGAGAAGGCAAGAGC |
| Internal bar code: | TGCGTACTTTCGGCCAGGACGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 761 |
| LEAP-Seq percent confirming: | 99.8178 |
| LEAP-Seq n confirming: | 548 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATCTACTCCAGCTCCGAC |
| Suggested primer 2: | GCTGTCAATGTGCTGGAAGA |