| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.215462 |
| Chromosome: | chromosome 9 |
| Location: | 1511584 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g398051 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGCTCACCATTTAACCTGTAGGTGCGCTC |
| Internal bar code: | CTCATGGGGGGGGTCAAATCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 754 |
| LEAP-Seq percent confirming: | 99.4674 |
| LEAP-Seq n confirming: | 747 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGGGAGGTTACACTTCGTA |
| Suggested primer 2: | TTTTGCATTGCTTCTTCGTG |