| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.215466 |
| Chromosome: | chromosome 2 |
| Location: | 6020503 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g112400 | CHI1 | (1 of 1) K12310 - Di-N-acetylchitobiase (CTBS); Chitinase-like hydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGTGGGCGACACACTGCATGCCACCACCG |
| Internal bar code: | GTCACGCAAAGGTAGATCGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 528 |
| LEAP-Seq percent confirming: | 99.7261 |
| LEAP-Seq n confirming: | 12745 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCCTCCCATAAGAGCAAA |
| Suggested primer 2: | GTTCCAATGTCTTGGCTGGT |