| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.215469 |
| Chromosome: | chromosome 9 |
| Location: | 2800902 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g388900 | TEH10 | Oleoyl-[acyl-carrier-protein] thioesterase/hydrolase; (1 of 1) PTHR11487//PTHR11487:SF0 - THIOESTERASE // S-ACYL FATTY ACID SYNTHASE THIOESTERASE, MEDIUM CHAIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGGTATTGCCCGCGGCGACTCATTCCGAG |
| Internal bar code: | GAGCCCTTACGTTGTAGGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 619 |
| LEAP-Seq percent confirming: | 98.8396 |
| LEAP-Seq n confirming: | 5366 |
| LEAP-Seq n nonconfirming: | 63 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAGCATTGGAAGCAGTGTC |
| Suggested primer 2: | GCTACTAGCGCCACCACTTC |