Insertion junction: LMJ.RY0402.215469_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.215469
Chromosome:chromosome 9
Location:2800918
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g388900 TEH10 Oleoyl-[acyl-carrier-protein] thioesterase/hydrolase; (1 of 1) PTHR11487//PTHR11487:SF0 - THIOESTERASE // S-ACYL FATTY ACID SYNTHASE THIOESTERASE, MEDIUM CHAIN intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCTGCCCCAACGCTCTTGGCTGCCGCTGC
Internal bar code:ACTTTTGCATCTCCGCATGTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:336
LEAP-Seq percent confirming:96.4868
LEAP-Seq n confirming:1538
LEAP-Seq n nonconfirming:56
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:TCAGCATTGGAAGCAGTGTC
Suggested primer 2:GCTACTAGCGCCACCACTTC