Insertion junction: LMJ.RY0402.215508_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.215508
Chromosome:chromosome 17
Location:563487
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g699750 CTL2 (1 of 1) IPR016187//IPR024616 - C-type lectin fold // Pherophorin; Putative pherophorin with C-type lectin 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTAGATGGGCTGATCCGGTCTTAGCGCGAG
Internal bar code:ACGACCCCTTGTCGTCGATCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:871
LEAP-Seq percent confirming:99.169
LEAP-Seq n confirming:716
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CGAGCCTGAAGACCTGATTC
Suggested primer 2:AGTGAAGGAGGAGGAGGAGC