| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.215519 |
| Chromosome: | chromosome 9 |
| Location: | 6772119 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g409350 | RLI1 | (1 of 1) K06174 - ATP-binding cassette, sub-family E, member 1 (ABCE1); Fe-S ABC transporter | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGCATCGGCAGCTCGGCAGTCGGGCGCG |
| Internal bar code: | AGCCATGGATGTCTGGCCCGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 664 |
| LEAP-Seq percent confirming: | 88.6454 |
| LEAP-Seq n confirming: | 890 |
| LEAP-Seq n nonconfirming: | 114 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCGTCTTACATTGGTCGTT |
| Suggested primer 2: | ACCCGTACTCCTCCACTCCT |