| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.215555 |
| Chromosome: | chromosome 12 |
| Location: | 225635 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g485100 | FAP326 | Flagellar Associated Protein 326; (1 of 2) PTHR20899//PTHR20899:SF1 - UNCHARACTERIZED // UPF0691 PROTEIN C9ORF116 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTCCAACGTTGTGCTCGCTGGCTTTCTGG |
| Internal bar code: | AGCCGCGTTTGGAAGGTTCTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 933 |
| LEAP-Seq percent confirming: | 99.176 |
| LEAP-Seq n confirming: | 7222 |
| LEAP-Seq n nonconfirming: | 60 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGTTGTGTGGAGGTCCTT |
| Suggested primer 2: | CTTGGCACCGTAGATGGAGT |