Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.215555 |
Chromosome: | chromosome_12 |
Location: | 225635 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre12.g485100 | sense | 5'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TCTCCAACGTTGTGCTCGCTGGCTTTCTGG |
Internal bar code: | AGCCGCGTTTGGAAGGTTCTTA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 933 |
LEAP-Seq percent confirming: | 99.176 |
LEAP-Seq n confirming: | 7222 |
LEAP-Seq n nonconfirming: | 60 |
LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGTGTTGTGTGGAGGTCCTT |
Suggested primer 2: | CTTGGCACCGTAGATGGAGT |