| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.215584 |
| Chromosome: | chromosome 12 |
| Location: | 2406763 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g507200 | (1 of 2) IPR000104//IPR003613 - Antifreeze protein, type I // U box domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTGCAGCAGTGGCGGGAGCGGCACCCCA |
| Internal bar code: | CACGCGGCTGCTTCCGCGTCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 259 |
| LEAP-Seq percent confirming: | 78.602 |
| LEAP-Seq n confirming: | 1653 |
| LEAP-Seq n nonconfirming: | 450 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTACCACAGACACGCACAC |
| Suggested primer 2: | GCTTTCGAGCAATGAACACA |