| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.215594 |
| Chromosome: | chromosome 2 |
| Location: | 2721719 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g093650 | (1 of 1) 1.14.12.22 - Carbazole 1,9a-dioxygenase / CARDO | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGATAATTGGCCATGGCAAATGCACATACC |
| Internal bar code: | GCCAGAAAAAGTGTGCCCGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 932 |
| LEAP-Seq percent confirming: | 99.7877 |
| LEAP-Seq n confirming: | 4230 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCGAGAAGAAATTCCAACA |
| Suggested primer 2: | GCAGACCACATTCGTCTCAA |