| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.215614 |
| Chromosome: | chromosome 10 |
| Location: | 1935378 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g432100 | (1 of 1) PTHR34687//PTHR34687:SF1 - FAMILY NOT NAMED // CHAPERONE PROTEIN DNAJ-LIKE PROTEIN | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGTAGAACTTGCGGCGCCCCTCGGCGGCC |
| Internal bar code: | ACCGATGTAATTCCCGGGGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 834 |
| LEAP-Seq percent confirming: | 99.5869 |
| LEAP-Seq n confirming: | 2652 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCGTAAGGACTGCATCAAT |
| Suggested primer 2: | GCACACCTTCTGGCTTTCTC |