| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.215634 |
| Chromosome: | chromosome 2 |
| Location: | 6050911 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g112600 | (1 of 3) PTHR13780//PTHR13780:SF49 - AMP-ACTIVATED PROTEIN KINASE, GAMMA REGULATORY SUBUNIT // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGCTGGGCAAGCCAGCACGCGTGTGCTC |
| Internal bar code: | GGGGGCAGCAATTGGGTTGGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 369 |
| LEAP-Seq percent confirming: | 96.7064 |
| LEAP-Seq n confirming: | 1703 |
| LEAP-Seq n nonconfirming: | 58 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTCCTTTGCGTCATTTGGT |
| Suggested primer 2: | GCTGCGAGTAGCCCAGATAC |