| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.215645 |
| Chromosome: | chromosome 17 |
| Location: | 6685507 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g744097 | GT90F49,GT90-49 | GT90 family protein 49; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCACTGGTCCCACGCGGCACGCGAAACA |
| Internal bar code: | ATAGTCCCGCTAATGCCTCTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1077 |
| LEAP-Seq percent confirming: | 99.7969 |
| LEAP-Seq n confirming: | 12282 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 64 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTCATGCTCTCGCCTTCAT |
| Suggested primer 2: | TTGTCTGACACCTTTGCAGC |