| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.215659 |
| Chromosome: | chromosome 14 |
| Location: | 828062 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g613150 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGTTAGGAAGAGCGGAAACGCGCGCGTG |
| Internal bar code: | TTTACGCAGATTAGCCACGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 341 |
| LEAP-Seq percent confirming: | 99.1933 |
| LEAP-Seq n confirming: | 18813 |
| LEAP-Seq n nonconfirming: | 153 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCCTTCCCCTTCTACTCC |
| Suggested primer 2: | GTGAACATGGGCTCGTAGGT |