| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.215684 |
| Chromosome: | chromosome 1 |
| Location: | 191911 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g001300 | ankryin repeat containing protein; (1 of 1) IPR000782//IPR006626 - FAS1 domain // Parallel beta-helix repeat | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTTCTCTCGCTCCTTCTCCCCATCCCTCT |
| Internal bar code: | CGGTATGGTCGTTTTCGGACACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 307 |
| LEAP-Seq percent confirming: | 75.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTTTCCTTTGGGACGTGT |
| Suggested primer 2: | CCGTACTGCACAACCATACG |