| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.215702 |
| Chromosome: | chromosome 17 |
| Location: | 2551713 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g716600 | (1 of 752) IPR027417 - P-loop containing nucleoside triphosphate hydrolase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCTCATGAGCGAGTACTCGGGACGGCCAG |
| Internal bar code: | AGGCTAAAATTGCAAGTGGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 766 |
| LEAP-Seq percent confirming: | 98.9891 |
| LEAP-Seq n confirming: | 12044 |
| LEAP-Seq n nonconfirming: | 123 |
| LEAP-Seq n unique pos: | 69 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTCTGTCGAGGCGGTAGTT |
| Suggested primer 2: | GCAAAGGTGGTTGGTTCAGT |