Insertion junction: LMJ.RY0402.215709_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.215709
Chromosome:chromosome 9
Location:1837096
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g395900 CSV3 Chlamydomonas-specific family protein 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCCCCCCCCGCAGTCTGAACCCCGGGCGC
Internal bar code:TTTACTAAACACTCGACTGTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:439
LEAP-Seq percent confirming:91.641
LEAP-Seq n confirming:8639
LEAP-Seq n nonconfirming:788
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:AGGGCGCATACCAATCATAC
Suggested primer 2:CACAACTACAACCCCCTGCT