| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.215724 |
| Chromosome: | chromosome 2 |
| Location: | 6736998 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g118350 | MFT14 | Major facilitator superfamily transporter; (1 of 82) IPR020846 - Major facilitator superfamily domain | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCCTGGAGCTTAGACCACCACCTGCGTCA |
| Internal bar code: | TCGAACGCACGAACACGTGCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1030 |
| LEAP-Seq percent confirming: | 99.2329 |
| LEAP-Seq n confirming: | 21604 |
| LEAP-Seq n nonconfirming: | 167 |
| LEAP-Seq n unique pos: | 70 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACATGACGAGTGGTGTGGAA |
| Suggested primer 2: | AGATACCAACCCTGCGACAC |