| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.215797 |
| Chromosome: | chromosome 17 |
| Location: | 3559299 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g725450 | (1 of 1) K11789 - HIV-1 Vpr-binding protein (VPRBP, DCAF1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTGAGGTGTGATATGATTGCGGCAATTG |
| Internal bar code: | GGGCTTTGTCGACTCACACTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1022 |
| LEAP-Seq percent confirming: | 99.5186 |
| LEAP-Seq n confirming: | 12611 |
| LEAP-Seq n nonconfirming: | 61 |
| LEAP-Seq n unique pos: | 96 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGATGAGGACATGGAGGA |
| Suggested primer 2: | GACTTGCGCTCCACTACTCC |