| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.215807 |
| Chromosome: | chromosome 4 |
| Location: | 1308079 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g214650 | BGS5,GSL1,GTR19 | Glucan synthase-like 1; (1 of 7) 2.4.1.34 - 1,3-beta-glucan synthase / UDP-glucose-1,3-beta-D-glucan glucosyltransferase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGTTCCCTTGCGCTCTGGCGCTTATAATG |
| Internal bar code: | AGCTAGGTGATGTATACTAGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 685 |
| LEAP-Seq percent confirming: | 99.5727 |
| LEAP-Seq n confirming: | 17711 |
| LEAP-Seq n nonconfirming: | 76 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACCGGTAGCCGAGGTTAGT |
| Suggested primer 2: | ATGGACGTAAAACCTGTGCC |