| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.215872 |
| Chromosome: | chromosome 10 |
| Location: | 5607370 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g460050 | FLU1,FLU,FLP | (1 of 1) PTHR10098//PTHR10098:SF123 - RAPSYN-RELATED // SUBFAMILY NOT NAMED; FLU protein, chloroplast precursor | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGTGGCGAAGCGAATAAAATTATTGGTG |
| Internal bar code: | GCACCTCATTATATTTTGCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 80 |
| LEAP-Seq percent confirming: | 99.0674 |
| LEAP-Seq n confirming: | 956 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCAGAGGATGCAGTCTGA |
| Suggested primer 2: | CACCAGGAGCTGAGCATACA |