| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.215882 |
| Chromosome: | chromosome 14 |
| Location: | 1650002 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g619050 | (1 of 1) PTHR31465:SF1 - PROTEIN RTA1-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGGATCAGTGCGACCAGGCCGCCGAGCGC |
| Internal bar code: | ATTGGCGTTCTTTAGTAGTGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 937 |
| LEAP-Seq percent confirming: | 99.4794 |
| LEAP-Seq n confirming: | 9936 |
| LEAP-Seq n nonconfirming: | 52 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGGGCTTACCTCCCTTAG |
| Suggested primer 2: | ATGAGCACGATGGTGGTGTA |