| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.215886 |
| Chromosome: | chromosome 12 |
| Location: | 5487660 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g530900 | (1 of 14) 3.6.3.25 - Sulfate-transporting ATPase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGATATTTACTGTGCGTGTCTTGGCGGA |
| Internal bar code: | CGACAGAACTCAACTAGGGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1085 |
| LEAP-Seq percent confirming: | 99.7199 |
| LEAP-Seq n confirming: | 17444 |
| LEAP-Seq n nonconfirming: | 49 |
| LEAP-Seq n unique pos: | 74 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGTGTTGAACCTTCACCTT |
| Suggested primer 2: | CTGGTTGATGACGCAGAAGA |