| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.215892 |
| Chromosome: | chromosome 6 |
| Location: | 1788418 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g262400 | (1 of 1) K14676 - lysophospholipid hydrolase (NTE, NRE) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTGGGCCTGCTGCCGCCGGTGTGGGAGGG |
| Internal bar code: | GACATAGTAGGTGTTGAAACTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 304 |
| LEAP-Seq percent confirming: | 95.6522 |
| LEAP-Seq n confirming: | 22 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCTGTTCATGTAGCTGCG |
| Suggested primer 2: | GTCTCCGGACTGCTACAAGC |