| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.215893 |
| Chromosome: | chromosome 3 |
| Location: | 2148710 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g157250 | (1 of 66) 2.7.12.1 - Dual-specificity kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGTGGGACCGCGACCGACCGACCGCCAA |
| Internal bar code: | GTTAGGTCGCTGGTTAGAATTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1111 |
| LEAP-Seq percent confirming: | 96.6772 |
| LEAP-Seq n confirming: | 4568 |
| LEAP-Seq n nonconfirming: | 157 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACGTAACGGCATGGTGAAA |
| Suggested primer 2: | ACGTCTAATGTCAGGGGCAC |