Insertion junction: LMJ.RY0402.215897_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.215897
Chromosome:chromosome 2
Location:3273711
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g095081 (1 of 1) PF09295 - ChAPs (Chs5p-Arf1p-binding proteins) (ChAPs) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AATGGGAACAGGATGCTAGCGGGGGAGCCA
Internal bar code:CTCAAGAGAGCGTCGCGTCCAC

Confirmation - LEAP-Seq

LEAP-Seq distance:363
LEAP-Seq percent confirming:99.6504
LEAP-Seq n confirming:3990
LEAP-Seq n nonconfirming:14
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:AATACCGGCAGTAGCAGCAC
Suggested primer 2:AGCTGGCTATAGGGTTGGGT