| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.215918 |
| Chromosome: | chromosome 2 |
| Location: | 1610390 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g085150 | (1 of 19) PTHR31251:SF1 - SQUAMOSA PROMOTER-BINDING-LIKE PROTEIN 10-RELATED | intron|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAATCCATTTTGGCAGGACCGAGCCCCTCA |
| Internal bar code: | TGCGTTCCTTGCTGACGATGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 984 |
| LEAP-Seq percent confirming: | 99.5759 |
| LEAP-Seq n confirming: | 9862 |
| LEAP-Seq n nonconfirming: | 42 |
| LEAP-Seq n unique pos: | 58 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAAGACCTGGGCTCTGAGT |
| Suggested primer 2: | GTGTGACCGCTGATACATGG |