| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.215920 |
| Chromosome: | chromosome 8 |
| Location: | 3632193 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g376250 | (1 of 7) IPR000104//IPR020683 - Antifreeze protein, type I // Ankyrin repeat-containing domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGTGTTCAGCGCCTGCGTCGGCAGCTGC |
| Internal bar code: | CGTCCGGGCATTAATATCTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 930 |
| LEAP-Seq percent confirming: | 98.4921 |
| LEAP-Seq n confirming: | 2874 |
| LEAP-Seq n nonconfirming: | 44 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATGAAGGCAGCAAAAATGG |
| Suggested primer 2: | TGCAGCTGCTGATGAAGAAC |