| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.215924 |
| Chromosome: | chromosome 11 |
| Location: | 1521890 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467748 | 3'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCGTCGCCCAACTCAGGAATACAGTGCGA |
| Internal bar code: | CCCGATCCGGGTCGCTTCGATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1098 |
| LEAP-Seq percent confirming: | 99.2286 |
| LEAP-Seq n confirming: | 15694 |
| LEAP-Seq n nonconfirming: | 122 |
| LEAP-Seq n unique pos: | 64 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTATAACAGCCACCACCGC |
| Suggested primer 2: | ATTCCGATCCTAGGCGAGAT |