| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.215932 |
| Chromosome: | chromosome 11 |
| Location: | 2914833 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g478000 | (1 of 18) IPR001041 - 2Fe-2S ferredoxin-type iron-sulfur binding domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTAATGTGCGGTGTTGCAACAGTCCTAC |
| Internal bar code: | ATGGTGACTCTACTGATACCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 703 |
| LEAP-Seq percent confirming: | 99.3641 |
| LEAP-Seq n confirming: | 24221 |
| LEAP-Seq n nonconfirming: | 155 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACACACGAGGACATCATCC |
| Suggested primer 2: | TTGTTGTATCTGGAGCAGCG |