| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.215934 |
| Chromosome: | chromosome 17 |
| Location: | 3333080 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g723400 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATACATGCTTGCAGCAGGTGGAGCCCTAC |
| Internal bar code: | GTTGAATGGCTATTGGGTATCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1100 |
| LEAP-Seq percent confirming: | 99.719 |
| LEAP-Seq n confirming: | 14549 |
| LEAP-Seq n nonconfirming: | 41 |
| LEAP-Seq n unique pos: | 67 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGACACACGTCCATTCACC |
| Suggested primer 2: | TGTACGTGACTGGGTACGGA |