| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.215955 |
| Chromosome: | chromosome 11 |
| Location: | 3313311 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g480200 | (1 of 4) IPR006597 - Sel1-like repeat | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCACCGGATCGCGTGCCGAGGCACCCCTG |
| Internal bar code: | GGGTTGCGCGAGGCACATTGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 988 |
| LEAP-Seq percent confirming: | 99.4673 |
| LEAP-Seq n confirming: | 8216 |
| LEAP-Seq n nonconfirming: | 44 |
| LEAP-Seq n unique pos: | 52 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCCTTGAAGTGGGGGTAGT |
| Suggested primer 2: | ATGTCCGTAGGCACTGGAAC |