| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.215959 |
| Chromosome: | chromosome 17 |
| Location: | 1999542 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g711250 | MIP2 | (1 of 2) PTHR19139:SF180 - AQUAPORIN-1; Aquaporin | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCGAGGGGTTGGTGAGAGCTGCTGGGGT |
| Internal bar code: | GTAAGCCTTATTTTCGGAGATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 902 |
| LEAP-Seq percent confirming: | 99.0967 |
| LEAP-Seq n confirming: | 18540 |
| LEAP-Seq n nonconfirming: | 169 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTTAAATGACGGTGCCTGG |
| Suggested primer 2: | GCTTGGTAACACATGCCCTT |