| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.215963 |
| Chromosome: | chromosome 14 |
| Location: | 911854 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g613600 | HSP70H | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACCGGTCGGTCTATCACCCAAACCCCGCG |
| Internal bar code: | TTACCTAAATGTAGCTTCGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 0 |
| LEAP-Seq percent confirming: | 93.1818 |
| LEAP-Seq n confirming: | 82 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACTGCTGCCACTGTTCCTG |
| Suggested primer 2: | AAGAGTGTGTGGATGAGGGG |